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KMID : 0385520080210010020
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Analytical Science & Technology
2008 Volume.21 No. 1 p.20 ~ p.24
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Determination of vitamin P₁ by chemiluminescence method
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Jin Seung-Oh
Lee Sang-Hak
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Abstract
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A chemiluminescence method has been developed to determine vitamin P₁in aqueous solution which is based on the enhancement of the intensity of lucigenin using lucigenin-H₂O₂as chemiluminogenic system. The effects of experimental parameters such as concentrations of lucigenin, pH and concentrations of H₂O₂ were studied. The present method allows the determination of vitamin P₁over the range 7.5×10?6~5.0×10?4 ㏖/L. The detection limit was 5.7×10?7 ㏖/L. The relative standard deviation was 0.75 % for 10 determinations of 7.5×10?5 ㏖/L vitamin P₁. The correlation coefficient of the working curve was 0.9984 (S/N=3).
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KEYWORD
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vitamin P₁, chemiluminescence, lucigenin
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